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KMID : 0616120090400010065
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Korean Journal of Pharmacognosy
2009 Volume.40 No. 1 p.65 ~ p.69
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The Effects of Litsea japonica on the Induction of Apoptosis in HL-60/ADR
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Kim Elvira
Boo Hye-Jin
Hyun Jae-Hee
Kim Sang-Cheol
Kang Jung-Il
Kim Min-Kyoung
Yoo Eun-Sook
Kang Hee-Kyoung
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Abstract
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The present study investigated the antiproliferative effect of Litsea japonica in HL-60/ADR, adriamycin resistant human promyelocytic leukemia cells. The 80 % ethanol extract of L. japonica markedly inhibited the growth of HL-60/ADR cells. When HL-60/ADR cells were treated with the extract, several apoptosis events like as DNA fragmentation, chromatin condensation and the increase of the population of sub-G1 hypodiploid cells were observed. In the mechanism of apoptosis induction by L. japonica, we examined the changes of Bcl-2 and Bax protein expression levels, and activation of caspases. After the HL-60/ADR cells were treated with the extract, the Bcl-2 expression was decreased, whereas the expression of Bax was increased in a time-dependent manner compared to the control. In addition, the active forms of caspase-9 and -3 were increased and the cleavage of poly (ADP-ribose) polymerase, a vital substrate of effector caspase, was observed. The results suggest that the inhibitory effect of L. japonica on the growth of the HL-60/ADR appears to arise from the induction of apoptosis via the down-regulation of Bcl-2 and the activation of caspases.
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KEYWORD
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Litsea japonica, HL-60/ADR, Apoptosis, Bcl-2, Caspases
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